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Original Research

Open Access

miR-187 modulates cardiomyocyte apoptosis and oxidative stress in myocardial infarction mice via negatively regulating DYRK2

  • Fen Zhu1
  • Zhili Yu1
  • Dongsheng Li1

1Department of Cardiology, Wuhan Third Hospital & Tongren Hospital of Wuhan University, 430060 Wuhan, Hubei, China

DOI: 10.22514/sv.2021.137 Vol.17,Issue 5,September 2021 pp.142-150

Submitted: 13 May 2021 Accepted: 30 July 2021

Published: 08 September 2021

*Corresponding Author(s): Fen Zhu E-mail: ADCB67iu95@163.com

Abstract

Myocardial infarction is a serious representation of cardiovescular disease, MicroRNAs play a role in modifying I/R injury and myocardial infarct remodeling. The present study therefore examined the potential role of miR-187 in cardiac I/R injury and its underlying mechanisms. miR-187 was inhibited or overexpressed in cardiomyocytes H/R models by pretreatment with miR-187 mimic or inhibitor to confirm the function of miR-187 in H/R. DYRK2 was inhibited or overexpressed in cardiomyocytes H/R models by pretreatment with DYRK2 inhibitor. A myocardium I/R mouse model was established. Circulating levels of miR-187 or DYRK2 was detected by quantitative realtime PCR and protein expression was detected by western blotting. The cell viability in all groups was determined by MTT assay and the apoptosis ratio was detected by flow cytometry after staining with Annexin V-FITC. The effect of miR-187 on cellular ROS generation was examined by DCFH-DA. The level of lipid peroxidation and SOD expression were determined by MDA and SOD assay. The findings indicated that miR-187 may be a possible regulator in the protective effect of H/R-induced cardiomyocyte apoptosis, cellular oxidative stress and leaded to DYRK2 suppression at a posttranscriptional level. Moreover, the improvement of miR-187 on H/R-induced cardiomyocyte injury contributed to the obstruction of DYRK2 expression. In addition, these results identified DYRK2 as the functional downstream target of miR-187 regulated myocardial infarction and oxidative stress.These present work provided the first insight into the function of miR-187 in successfully protect cardiomyocyte both in vivo and in vitro, and such a protective effect were mediated through the regulation of DYRK2 expression.


Keywords

miR-187; DYRK2; Cardiomyocyte; Myocardial infarction; Oxidative stress


Cite and Share

Fen Zhu,Zhili Yu,Dongsheng Li. miR-187 modulates cardiomyocyte apoptosis and oxidative stress in myocardial infarction mice via negatively regulating DYRK2. Signa Vitae. 2021. 17(5);142-150.

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