Article Data

  • Views 375
  • Dowloads 150

Original Research

Open Access

Methyl jasmonate reduces the inflammation and apoptosis of HK-2 cells induced by LPS by regulating the NF-κB pathway

  • Fuhai Chen1
  • Wei Wang2
  • Xianguo Cai1
  • Hongsheng Yu3
  • Chunsheng Qu2
  • Xianjun Zhang1
  • Leili Lv2
  • Zhichun Song2

1Department of Urinary Surgery, Taizhou Hospital of Zhejiang Province, Zhejiang University, 317000 Taizhou City, Zhejiang Province, China

2Department of Clinical Laboratory, Lishui People’s Hospital, 323000 Lishui City, Zhejiang Province, China

3Department of Clinical Laboratory, Wuhan Children’s Hospital, 430016 Wuhan City, Hubei Province, China

DOI: 10.22514/sv.2021.079 Vol.17,Issue 3,May 2021 pp.218-224

Submitted: 19 February 2021 Accepted: 23 March 2021

Published: 08 May 2021

*Corresponding Author(s): Zhichun Song E-mail: songzhichun10209@163.com

Abstract

Background: Methyl jasmonate is a bioactive oxylipid that participates in the defense-related mechanisms of plants. The anti-inflammatory and anti-oxidative capacities of methyl jasmonate against lipopolysaccharide (LPS) induced arthritis have been widely investigated. However, the role of methyl jasmonate in LPS-induced cell model of tubular-interstitial nephritis (TIN) has not been reported.

Methods: LPS (5 µg/mL) was applied to treat human renal tubular epithelial cell line (HK-2) for the establishment of TIN cell model. LPS-induced HK-2 was incubated with 10 or 20 µM methyl jasmonate, cell viability and apoptosis were assessed by MTT and flow cytometry. ELISA and qRT-PCR were performed to determine the levels of interleukin (IL)-1 beta (IL-1β), IL-6, IL-8 and tumor necrosis factor-α (TNF-α). The downstream pathway was investigated by western blot.

Results: LPS induced cytotoxicity in HK-2 cell accompanied by decrease of cell viability and increase of cell apoptosis. Methyl jasmonate dosage dependently enhanced the cell viability and reduced cell apoptosis to ameliorate the cytotoxicity. LPS also induced inflammatory response in HK-2 cell with increased IL-1β, IL-6, IL-8 and TNF-α. Methyl jasmonate attenuated LPS-induced inflammation in HK-2 cell. Protein expression of IκBα was down-regulated, p65 and IκBα phosphorylation were up-regulated in LPS-induced HK-2. Methyl jasmonate attenuated LPS-induced decrease of IκBα and increase of p65 and IκBα phosphorylation in HK-2 cell.

Conclusion: Methyl jasmonate demonstrated anti-apoptotic and anti-inflammatory effects on LPS-induced HK-2 cell through suppression of NF-κB activation.


Keywords

Methyl jasmonate; LPS; Ochratoxin A; Apoptosis; Inflammation; NF-κB


Cite and Share

Fuhai Chen,Wei Wang,Xianguo Cai,Hongsheng Yu,Chunsheng Qu,Xianjun Zhang,Leili Lv,Zhichun Song. Methyl jasmonate reduces the inflammation and apoptosis of HK-2 cells induced by LPS by regulating the NF-κB pathway. Signa Vitae. 2021. 17(3);218-224.

References

[1] Joyce E, Glasner P, Ranganathan S, Swiatecka-Urban A. Tubuloin-terstitial nephritis: diagnosis, treatment, and monitoring. Pediatric Nephrology. 2017; 32: 577–587.

[2] Zhang P, Cornell LD. IgG4-related tubulointerstitial nephritis. Advances in Chronic Kidney Disease. 2017; 24: 94–100.

[3] Rodríguez-Iturbe B, Johnson RJ, Herrera-Acosta J. Tubulointerstitial damage and progression of renal failure. Kidney International Supple-ments. 2005; S82–S86.

[4] Dos Santos Haupenthal DP, Mendes C, de Bem Silveira G, Zaccaron RP, Corrêa MEAB, Nesi RT, et al. Effects of treatment with gold nanoparticles in a model of acute pulmonary inflammation induced by lipopolysaccharide. Journal of Biomedical Materials Research Part A. 2020; 108: 103–115.

[5] Eardley KS, Zehnder D, Quinkler M, Lepenies J, Bates RL, Savage CO, et al. The relationship between albuminuria, MCP-1/CCL2, and interstitial macrophages in chronic kidney disease. Kidney International. 2006; 69: 1189–1197.

[6] Shui H, Ka S, Wu W, Lin Y, Hou Y, Su L, et al. LPS-evoked IL-18 expression in mesangial cells plays a role in accelerating lupus nephritis. Rheumatology. 2007; 46: 1277–1284.

[7] Pereira-Maróstica HV, Castro LS, Gonçalves GA, Silva FMS, Bracht L, Bersani-Amado CA, et al. Methyl jasmonate reduces inflammation and oxidative stress in the brain of arthritic rats. Antioxidants. 2019; 8: 485.

[8] Cesari IM, Carvalho E, Figueiredo Rodrigues M, Mendonça BDS, Amôedo ND, Rumjanek FD. Methyl jasmonate: putative mechanisms of action on cancer cells cycle, metabolism, and apoptosis. International Journal of Cell Biology. 2014; 2014: 572097.

[9] Alabi AO, Ajayi AM, Ben-Azu B, Bakre AG, Umukoro S. Methyl jas-monate abrogates rotenone-induced parkinsonian-like symptoms through inhibition of oxidative stress, release of pro-inflammatory cytokines, and down-regulation of immnopositive cells of NF-κB and α-synuclein expressions in mice. NeuroToxicology. 2019; 74: 172–183.

[10] Sá-Nakanishi AB, Soni-Neto J, Moreira LS, Gonçalves GA, Silva FMS, Bracht L, et al. Anti-inflammatory and antioxidant actions of methyl jasmonate are associated with metabolic modifications in the liver of arthritic rats. Oxidative Medicine and Cellular Longevity. 2018; 2018: 2056250.

[11] Gunjegaonkar SM, Wankhede SB, Shanmugarajan TS, Shinde SD. Bioactive role of plant stress hormone methyl jasmonate against lipopolysaccharide induced arthritis. Heliyon. 2020; 6: e05432.

[12] Boozari M, Hosseinzadeh H. Natural medicines for acute renal failure: a review. Phytotherapy Research. 2017; 31: 1824–1835.

[13] Umukoro S, Alabi AO, Eduviere AT, Ajayi AM, Oluwole OG. Anti-inflammatory and membrane stabilizing properties of methyl jasmonate in rats. Chinese Journal of Natural Medicines. 2017; 15: 202–209.

[14] Tanaka T, Nangaku M. Pathogenesis of tubular interstitial nephritis. Contributions to Nephrology. 2011; 169: 297–310.

[15] Zhi D, Zhang M, Lin J, Liu P, Duan M. GPR120 ameliorates apoptosis and inhibits the production of inflammatory cytokines in renal tubular epithelial cells. Inflammation. 2021; 44: 493–505.

[16] Petrik J, Zanić-Grubisić T, Barisić K, Pepeljnjak S, Radić B, Ferencić Z, et al. Apoptosis and oxidative stress induced by ochratoxin a in rat kidney. Archives of Toxicology. 2003; 77: 685–693.

[17] Mao H, Li Z, Zhou Y, Li Z, Zhuang S, An X, et al. HSP72 attenuates renal tubular cell apoptosis and interstitial fibrosis in obstructive nephropathy. American Journal of Physiology-Renal Physiology. 2008; 295: F202–F214.

[18] Li J, Chen K, Wang F, Dai W, Li S, Feng J, et al. Methyl jasmonate leads to necrosis and apoptosis in hepatocellular carcinoma cells via inhibition of glycolysis and represses tumor growth in mice. Oncotarget. 2017; 8: 45965–45980.

[19] Viatour P, Merville M, Bours V, Chariot A. Phosphorylation of NF-κB and IκB proteins: implications in cancer and inflammation. Trends in Biochemical Sciences. 2005; 30: 43–52.

[20] Zheng L, Sinniah R, Hsu SI. Pathogenic role of NF-κB activation in tubulointerstitial inflammatory lesions in human lupus nephritis. Journal of Histochemistry & Cytochemistry. 2008; 56: 517–529.

[21] Chen Y, Zheng Y, Zhou Z, Wang J. Baicalein alleviates tubular-interstitial nephritis in vivo and in vitro by down-regulating NF-κB and MAPK pathways. Brazilian Journal of Medical and Biological Research. 2018; 51: e7476.

[22] Cesari IM, Carvalho E, Figueiredo Rodrigues M, Mendonça BDS, Amôedo ND, Rumjanek FD. Methyl jasmonate: putative mechanisms of action on cancer cells cycle, metabolism, and apoptosis. International Journal of Cell Biology. 2014; 2014: 572097.

[23] Sabry A, Elbasyouni SR, Sheashaa HA, Alhusseini AA, Mahmoud K, George SK, et al. Correlation between levels of TNF-α and IL-6 and hematological involvement in SLE Egyptian patients with lupus nephritis. International Urology and Nephrology. 2006; 38: 731–737.

[24] Anjum SA, Wang L, Farooq M, Khan I, Xue L. Methyl jasmonate-induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought. Journal of Agronomy and Crop Science. 2011; 197: 296–301.

[25] Li H, Lv L, Wu C, Qi J, Shi B. Methyl jasmonate protects microglial cells against β-amyloid-induced oxidative stress and inflammation via Nrf2-dependent HO-1 pathway. Neuropsychiatric Disease and Treatment. 2020; 16: 1399–1410.

[26] Mousavi SR, Niknejad Y, Fallah H, Tari DB. Methyl jasmonate alleviates arsenic toxicity in rice. Plant Cell Reports. 2020; 39: 1041–1060.

[27] Pereira-Maróstica HV, Castro LS, Gonçalves GA, Silva FMS, Bracht L, Bersani-Amado CA, et al. Methyl jasmonate reduces inflammation and oxidative stress in the brain of arthritic rats. Antioxidants. 2019; 8: 485.


Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Chemical Abstracts Service Source Index The CAS Source Index (CASSI) Search Tool is an online resource that can quickly identify or confirm journal titles and abbreviations for publications indexed by CAS since 1907, including serial and non-serial scientific and technical publications.

IndexCopernicus The Index Copernicus International (ICI) Journals database’s is an international indexation database of scientific journals. It covered international scientific journals which divided into general information, contents of individual issues, detailed bibliography (references) sections for every publication, as well as full texts of publications in the form of attached files (optional). For now, there are more than 58,000 scientific journals registered at ICI.

Geneva Foundation for Medical Education and Research The Geneva Foundation for Medical Education and Research (GFMER) is a non-profit organization established in 2002 and it works in close collaboration with the World Health Organization (WHO). The overall objectives of the Foundation are to promote and develop health education and research programs.

Scopus: CiteScore 0.5(2019) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Embase Embase (often styled EMBASE for Excerpta Medica dataBASE), produced by Elsevier, is a biomedical and pharmacological database of published literature designed to support information managers and pharmacovigilance in complying with the regulatory requirements of a licensed drug.

Submission Turnaround Time

Conferences

Top