Article Data

  • Views 1083
  • Dowloads 158

Original Research

Open Access

Immature granulocyte count on the new Sysmex XN-9000: performance and diagnosis of sepsis in the intensive care unit

  • SABRINA BUORO1
  • TOMMASO MECC1
  • MAURO VAVASSO1
  • GIOVANNA AZZA1
  • SARA APASSITI ESPOS1
  • PAOLA DOMINONI1
  • GABRIELE PAPAGN1
  • GIANMARIANO MARCHES1
  • COSIMO OTTOMAN1
  • ALBERTO CRIP1
  • GIUSEPPE LI1

1,USC Laboratorio Analisi Chimico Cliniche A.O. Papa Giovanni XXIII

DOI: 10.22514/SV102.122015.4 Vol.10,Issue 2,December 2015 pp.54-65

Published: 14 December 2015

*Corresponding Author(s): SABRINA BUORO E-mail: sbuoro@hpg23.it

Abstract

quantified as the band or immature granulocyte (IG) count. The aim of this study was to analyze the IG count performance of the Sysmex XN-9000 hematology analyzer in intensive care unit (ICU) patients.

Methods. 480 peripheral blood samples from adult patients admitted to the ICU (301 control, 119 sepsis and 60 septic shock) were analyzed with Sysmex XN-9000. Serum C reactive protein (CRP) was measured on Siemens ADVIA 2400. IG count in peripheral blood was determined either by XN-9000 or optical microscopy (OM). Agreement between the two methods was assessed with Pearson’s correlation, Passing-Bablok regression and Bland Altman bias. Diagnostic accuracy was estimated through ROC curves analysis. Sysmex XN-9000 imprecision and within-run precision were also evaluated.

Results. Pearson’s correlation (r) relative to IG count, as absolute and percentage values, was 0.89 (p <0.0001) and 0.74 (p <0.0001), respectively, with a Bias of 0.22 and 1.69 respectively. The Area Under the curve (AUC) for the IG count for diagnosing sepsis was greater on XN-9000 than OM and equal to the serum CRP. The diagnostic accuracy of IG counts improves when taking into account the conventional criteria for diagnosing sepsis.

Conclusion. IG count appears suitable and reliable when performed using XN-9000. Even if a modest overestimation was found, the diagnostic accuracy showed by IG analysis on XN-9000 may represent a valid alternative to OM count for diagnosing sepsis in ICU patients.

Keywords

immature granulocyte, Sysmex XN, sepsis, automated cell count

Cite and Share

SABRINA BUORO,TOMMASO MECC, MAURO VAVASSO,GIOVANNA AZZA,SARA APASSITI ESPOS,PAOLA DOMINONI,GABRIELE PAPAGN,GIANMARIANO MARCHES,COSIMO OTTOMAN,ALBERTO CRIP,GIUSEPPE LI. Immature granulocyte count on the new Sysmex XN-9000: performance and diagnosis of sepsis in the intensive care unit. Signa Vitae. 2015. 10(2);54-65.

References

1. Nguyen HB, Rivers EP, Abrahamian FM, Moran GJ, Abraham E, Trzeciak S, et al. Severe sepsis and septic shock: rewiew of the literature and emergency department management guidelines”. Ann Emerg Med 2006;48:28-54.

2. Martin GS, Mannino DM, Eaton S, Moss M. The epidemiology of sepsis in the United States from 1979 through 2000. N Engl J Med 2003;348:1546-54.

3. Angus DC, van Der Poll T. Severe sepsis and septic shock. N Engl J Med 2013;369:840-51.

4. Levy MM, Fink MP, Marshall JC, Abraham E, Angus D, Cook D, et al. International Sepsis Definitions Conference. Crit Care Med 2003;4:1250-6.

5. van der Meer W, van Gelder W, de Keijzer R, Willems H. Does the band cell survive the 21st century? Eur J Haematol 2006:76:251–4.

6. Fernandes B, Hamaguchi Y. Automated enumeration of immature granulocytes. Am J Clin Pathol 2007;128:454-63.

7. Nigro KG, O’Riordan MA, Molloy EJ, Walsh MC, Sandhaus LM. Performance of an automated immature granulocyte count as a predictor of neonatal sepsis. Am J Clin Pathol 2005;123:618-24.

8. Ansari-Lari MA, Kickler TS, Borowitz MJ. Immature granulocyte measurement using the Sysmex XE-2100 relationship to infection and sepsis. Am J Clin Pathol 2003;120:795-9.

9. Maenhout TM, Marcelis L. Immature granulocyte count in peripheral blood by the Sysmex haematology XN Series Compared to microscopic differentiaton. J Clin Pathol 2014; 67:648-50.

10. Bernstein LH, Rucinsky J. Measurement of granulocyte maturation may improve the early diagnosis of the septic state. Clin Chem Lab Med 2011;49:2089-95.

11. Briggs C, Longair I, Kumar P, Singh D, Machin SJ. Performance evaluation of the Sysmex haematology XN modular system. J Clin Pathol 2012;65:1024-30.

12. Tantanate C. Spurious white blood cell count from a new automated Sysmex XN hematology analyzer. Int J Lab Hematol 2014;36(6): 86-7.

13. Koepke JA, van Assendelft OW, Brindza LJ, Davis BH, Fernandes BJ, Gewirtz AS, et al. Reference Leukocyte (WBC) Differential Count (Proportional) and Evaluation of instrumental Methods – Approved Standard, second ed. CLSI document H20A2, Wayne, PA, 2010.

14. College of American Pathologists. Hematology and Clinical Microscopy Glossary, Illinois, 2012, pp. 3–5.

15. Barnes PW, McFadden L, Machin SJ, Simson E. The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis. Lab Hematol 2005;11:83-90.

16. McEnroe RJ, Durham AP, Goldford MD, Kondratovich MV, Lababidi S, Magari R, Middle JG, Pierson-Perry JF, Vaks JE. Evaluation of Precision of Quantitative Measurement Procedures – Approved Guideline, third edition. CLSI guideline EP05-A3E, Wayne, PA, 2014.

17. Park SH, Park CJ, Lee BR, Nam KS, Kim MJ, Han MY, et al. Sepsis affects most routine and cell population data

(CPD) obtained using the Sysmex XN-2000 blood cell analyzer: neutrophil-related CPD NE-SFL and NE-WY provide useful information for detecting sepsis. Int J Lab Hematol 2014 May 28. doi: 10.1111/ijlh.12261.

Abstracted / indexed in

Science Citation Index Expanded (SciSearch) Created as SCI in 1964, Science Citation Index Expanded now indexes over 9,200 of the world’s most impactful journals across 178 scientific disciplines. More than 53 million records and 1.18 billion cited references date back from 1900 to present.

Journal Citation Reports/Science Edition Journal Citation Reports/Science Edition aims to evaluate a journal’s value from multiple perspectives including the journal impact factor, descriptive data about a journal’s open access content as well as contributing authors, and provide readers a transparent and publisher-neutral data & statistics information about the journal.

Chemical Abstracts Service Source Index The CAS Source Index (CASSI) Search Tool is an online resource that can quickly identify or confirm journal titles and abbreviations for publications indexed by CAS since 1907, including serial and non-serial scientific and technical publications.

IndexCopernicus The Index Copernicus International (ICI) Journals database’s is an international indexation database of scientific journals. It covered international scientific journals which divided into general information, contents of individual issues, detailed bibliography (references) sections for every publication, as well as full texts of publications in the form of attached files (optional). For now, there are more than 58,000 scientific journals registered at ICI.

Geneva Foundation for Medical Education and Research The Geneva Foundation for Medical Education and Research (GFMER) is a non-profit organization established in 2002 and it works in close collaboration with the World Health Organization (WHO). The overall objectives of the Foundation are to promote and develop health education and research programs.

Scopus: CiteScore 0.5(2019) Scopus is Elsevier's abstract and citation database launched in 2004. Scopus covers nearly 36,377 titles (22,794 active titles and 13,583 Inactive titles) from approximately 11,678 publishers, of which 34,346 are peer-reviewed journals in top-level subject fields: life sciences, social sciences, physical sciences and health sciences.

Embase Embase (often styled EMBASE for Excerpta Medica dataBASE), produced by Elsevier, is a biomedical and pharmacological database of published literature designed to support information managers and pharmacovigilance in complying with the regulatory requirements of a licensed drug.

Submission Turnaround Time

Conferences

Top